Plant pigments with phyto-compounds are used for synthetizing nanoparticles. These pigments were used to compare the efficacy of silver nanoparticles synthesized from betanin pigment extracted from Beta vulgaris L. and Amaranthus tricolor L. The silver nanoparticles and betanin related derivatives were synthesized from the pigment and analysed using UV-Vis spectrophotometry. The SEM images of nanoparticles showed the appearance of spherical shape with definite size. The FTIR analysis was carried out to identify possible biomolecules responsible in bio-reduction of silver ions and compare their compounds. FTIR and UV-Vis spectra showed that biomolecules, proteins and peptides, are mainly responsible for the formation and stabilization of AgNPs. These molecules interact with Phyto-compounds and form a stabilized complex. The antibacterial activity using resazurin dye showed 6.25±0.03 µg/ µl inhibition of Bacillus sp. and E.coli strains by the silver nanoparticles synthesized from B. vulgaris. Both gram positive and gram negative bacteria, Bacillus sp. and E.coli strains were used to measure the MICs. Resazurin dye was used to for the detection of the inhibitory level of nanoparticles synthesized pigments from Beta vulgaris L. and Amaranthus tricolor L.The study confirms abundant silver nanoparticles seen on B. vulgaris when compared to A. tricolor. Also, the Betanin pigment extracted from natural source showed antimicrobial property could be a promising molecule for new innovation in pharmaceutical sciences. The crude and silver nanoparticle pigment derived from plants could be used in textile industry for making the antibacterial garments. Further, it can be applied as a coating agent for capsule preparation in medicinal field.