Over the past century, the area of plant tissue culture has made remarkable advancements by embracing new technologies and ideas. To reduce the dependency on natural resources, scientists have worked on creating plants artificially. In response to the desire for high-yielding and disease-resistant cultivars, large-scale production methods that can create thousands of plants from a single explant have been created. Globalization has driven the commercialization of PTC worldwide, particularly due to the increasing demand from developing countries. Modified micropropagation techniques have been effectively applied in forestry, medicine, and agriculture in India by the PTC industry. In situ conservation, germplasm sharing, pilot trials, and protocol improvement are some strategies that have revolutionized the Indian PTC market, contributing to its significant progress. Ensuring food security is crucial, and although India produces enough cereals, there is a shortage of pulses and oilseeds. To tackle this, promoting crop diversification and improving related sectors are necessary.