Wheatgrass is the world's most edible grain cereal-grass which belongs to the Gramineae (Poaceae) family. It is rich in vitamins and proteins in comparison of seed kernel, or grain products. Wheatgrass is effective in the treatment for several diseases like ulcerative colitis, cancer, mouth ulcers and joint pain etc. Present study was carried out in order to analyse the amino acid and vitamin content of wheatgrass powder. For that, wheatgrass was soaked, germinated and grown in plastic trays and dried in microwave at 600 W for 15 mins. Then, fine powder was formed. Amino acids namely methionine, lysine, leucine, phenylalanine, valine, histidine, isoleucine, threonine and tryptophan were analysed by HPLC. β carotene and Ascorbic acid were analysed by AOAC (2000) whereas folic acid was analysed by the method of Ranganna (1986). It was found that wheatgrass powder had methionine, lysine, leucine, phenylalanine, valine, histidine, isoleucine, threonine and tryptophan were present in the amount of 64.4, 73.42, 120.39, 112.13, 116.33, 36.44, 80.18, 96.2, 35.32 mg/ 100g respectively. Whereas, amount of β carotene was 182.46 ± 0.040 µg, ascorbic acid was 2.25 ± 0.005 mg and folic acid was 212.92 ± 0.078 µg present in wheatgrass powder.