This is a clinical study to evaluate the efficacy of Vettummaaran Kuligai (VMK) in comparison with an allopathic drug (Amikacin) for patients affected with mild and moderate fever. In this study, the antimicrobial effect and Minimum Inhibitory Concentration (MIC) of Seed extract with different clinical isolates and their successive comparison with commercially available antibiotics were done. The bacterial growth was inhibited by the Disk diffusion method. This in vitro analysis was done with various clinical isolates such as Bacillus subtilis, Klebsiella, Proteus mirabilis, Salmonella typhi, Enterococcus, E.coli, Seeatiamarcens, Pseudomonas aeruginosa, and Staphylococcus aureus. The best MIC values of the isolates were recorded at different dilutions of seed extract. The zone of inhibition was measured and the sensitivity and resistance of the organisms were determined. The results showed that lower dilutions of drug suspension have more susceptibility than the higher dilutions for all the clinical isolates. The results of the present study propose that lower doses of Vettumaran Kuligai will be more effective. Further exploration of the antimicrobial effects of the Vettumaran Kuligai drug against various bacterial strains should be done in detail.