Introduction: Breast cancer refers to cancer occurring in breast in which the cells grow abnormally as they grow and divide in nature uncontrolled way. This type is the second most common cancer in the whole world affecting the female population leading to death. The aim of the study was to assess the knowledge regarding breast cancer among Nursing students of selected nursing college at Gurugram. Methodology: A quantitative research approach adopted for this study and descriptive research design was used. This study was conducted on 60 nursing students of selected nursing college at Gurugram, nonprobability convenient sampling technique was used. The data of the study were collected by use of valid tool which comprise of demographic variables and self-structured knowledge questionnaires. The data analysis process was done with SPSS 20 Result: The result of the study revealed that the large portion of the participants 54 (90%) had moderate level of knowledge and 6(10%) had inadequate level of knowledge and no one had adequate knowledge. In terms of association, no association were found between knowledge score and demographic variables. Conclusion: This study highlighted that nursing students have moderate level of knowledge regarding breast cancer. A structured teaching program or wide skill based curriculum is needed for positive outcomes on prevention of breast cancer.