This study was conducted to assess the effectiveness of self instructional module on use of aseptic technique among staff nurses. The main objective of study was to assess the knowledge on aseptic technique among staff nurses in district hospital shahjahanpur (u.p.) A quantitative research approach was used to evaluate the knowledge regarding use of aseptic technique during patient care among staff nurses working in district hospital shahjahanpur U.P. and to find out the association between knowledge regarding use of aseptic technique during patient care among staff nurses with their selected demographic variables.