Adolescents are a heterogeneous group with different and evolving needs, depending on their personal development stages and life circumstance as they transition from childhood, through adolescence into adulthood, all individuals must be prepared with the knowledge and skills they need to make use of the opportunities and to face the challenges they will encounter in the adult world. These efforts should contribute to building their sense of self-worth and to strengthen their links with the individuals and institutions in their communities. However, in many places they feel unprepared and unable to address the sensitive matters around puberty, sexuality and reproduction. The aim of the present study is to measure the effectiveness of the reproductive intervention programme among adolescent girls and to evaluate the knowledge of reproductive health before and after the intervention programme with reference to stream of education. The sample for the present study was selected by random sampling method and quasi-experimental pre-test, post-test research design was conducted. A total of 120 subjects were chosen, consisting of 60 adolescent girls from arts and 60 adolescent girls from science stream. The tool administered were questionnaires formulated by the investigator. Intervention was delivered using lectures, discussions, and demonstration by posters, videos, printed material, over-head projectors, black board and discussions. The collected data was statistically analyzed by Mean, Standard Deviation, t- test. The conclusion drawn from the present study revealed that, adolescent girls of science stream had more knowledge about reproductive health before intervention compared to the adolescent girls of arts steam. After intervention, adolescent girls of science stream showed more knowledge about reproductive health in comparison to adolescent girls of arts stream. Thus informative and educable interventions seem to have a positive effect on awareness levels which would eventually encourage expansion of knowledge about reproductive health and positive health habits.