Important issues in using biotechnology in agriculture and forestry are for example to enhance productivity and stress resistance of crops and trees, mainly due to restricted land area and increasing environmental pressures, and to develop carbon dioxide-neutral production systems for sustainable production of fiber/biomass and biofuel with biotechnological methods Along with the production issues, we need to conserve and protect natural diversity and species richness as a foundation of life on earth. With the recognition that increased plant diversity may also increase productivity, especially at low resource input, novel production systems combining aspects of diversity and biotechnology are emerging. Ecosystem and Forestry was an important to human beings. The techniques of plant, organ, tissue and cell culture have evolved since the beginning of this century and combined with recent advances in genetics and using conventional plant breeding, the plant biotechnology is having a significant impact on agriculture, horticulture and forestry. Some examples of the current applications in agriculture are micropropagation, somatic embryogenesis, virus and pathogen elimination, embryo rescue, germplasm storage and plant modification by somaclonal variation and genetic engineering. Another significant potential of plant biotechnology is in vitro production of fine chemicals using plant cell/organ cultures. This research article is to be discussed about the A study on Ecosystem and Horticulture Forestry.